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TAO Li LAN Xian-li LI Jun MA Chun-xia LAN Jin-hong HUANG Ming-xue LAN Mei-yi BI Bing-fen CHEN Ze-xiang YANG Wei 《中国畜牧兽医》2016,43(4):1072-1078
To investigate the pathogens of goat's respiratory disease from a goat farm in Guangxi province, the epidemiological investigation, clinic observation, pathological examination, bacterium isolation and identification, biochemical test, PCR and animal experiment test were conducted.The preventive and control measures were taken on the basis of pathogen epidemiological characteristics and drug sensitive test results.The results showed that Mycoplasma, influenza virus and parasite were negative by isolation and culture or PCR, however, two strains of gram-negative bacteria named MS1 and MS2 were isolated from lung.MS1 was identified as Serratia marcescens by biochemical test and 16S rRNA sequencing, which shared 99% nucleotide homology with other Serratia marcescens in GenBank.And MS2 was identified as E.coil by the same methods that shared 99% nucleotide homology with other E.coli in GenBank.Animal experiment showed that two strains could cause death in mice.The drug sensitive tests showed two strains were highly sensitive to spectinomycin, amikacin, kanamicin and neomycin.Kanamycin and dexamethasone were used to treat the sick goats, and achieved good results. 相似文献
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Plant leucine-rich repeat (LRR) domain-containing proteins are known to play important roles in signaling transduction and defense responses. In sorghum, SbLRR2 is pathogen-inducible gene encoding a simple extracellular LRR protein. Here, we demonstrated an earlier and stronger expression of SbLRR2 in a sorghum resistant genotype in comparison to a susceptible genotype following inoculation with the anthracnose pathogen (Colletotrichum sublineolum). In addition, SbLRR2 expression was found to be induced strongly by methyl-jasmonate treatment. Functional analysis was performed in SbLRR2 over-expression (OE) Arabidopsis plants, which showed enhanced resistance against the necrotrophic pathogens Botrytis cinerea and Alternaria brassicicola. In addition, the OE lines were found to have elevated expression of several jasmonate acid (JA)-associated genes and higher endogenous JA contents. Hence, the SbLRR2-mediated defense responses in transgenic Arabidopsis are likely to be dependent on JA-signaling through increased JA production. On the other hand, the OE lines remained susceptible to Pseudomonas syringae pv. tomato like the wild type plants. Consistently, there was no up-regulation of salicylic acid (SA) defense marker gene expression or SA levels in the OE lines. Our results suggested that SbLRR2 is potentially useful for enhancing resistance against necrotrophic pathogens in transgenic dicot crops. 相似文献
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Xiaojie Liu Xiaoshuang Li Tohir A. Bozorov Rong Ma Jinbiao Ma Yiheng Zhang Honglan Yang Li Li Daoyuan Zhang 《Forest Pathology》2020,50(3)
Cytospora species are capable of causing destructive cankers of stems belonging to a wide range of woody plant species. In severe cases, cankers may lead to dieback of twigs and branches. Little is known about the Cytospora species causing canker disease of wild apple (Malus sieversii) trees in the Wild Fruit Forest Reserve in Tianshan Forest, Xinjiang Uygur Autonomous Region, China. In this study, six Cytospora isolates belonging to two species were isolated from cankerous lesions of wild apple twigs. Based on multi‐locus phylogenetic analysis using three DNA markers (ITS, tef1‐α and tub2) and morphological characterization, these isolates were identified as Cytospora mali and Cytospora parasitica. Temperature trials (15, 20, 25 and 30°C) showed that the optimal growth temperature for six isolates was 25°C. At a variety of temperatures, C. mali isolates tended to grow faster than isolates of C. parasitica, with the C. mali isolate, EGI1 performing better than others with regard to growth rate. Morphological observations showed that these species exhibited a single locule without conceptacles, and the conidia length was 3–5 μm. In vitro inoculation trials of twigs and leaves of M. sieversii seedlings revealed that the C. mali isolates were highly virulent phytopathogenic fungi, whereas the C. parasitica isolates were less virulent. The isolate EGI1 was the most virulent isolate among the six isolates. This paper presents the first report of pathogenic Cytospora spp. of the M. sieversii Tianshan Wild Fruit Forest Reserve of Yili, Xinjiang in China. It will aid in the understanding of how apple tree cankers are induced and provide disease management guidelines for M. sieversii forest conservation. 相似文献
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为了探讨PRC2复合体在铁皮石斛生长发育和胁迫响应中的功能,通过生物信息学方法筛选了铁皮石斛PRC2核心成员DcCLF、DcSWN、DcEMF2、DcFIE和DcMSI1,借助酵母双杂交技术分析了它们之间的互作关系,利用半定量PCR分析了PRC2核心成员的组织表达谱,通过荧光定量PCR检测了它们对非生物胁迫(低温、高温、脱水)和病害胁迫(齐整小核菌、灰葡萄孢霉菌)的响应情况。结果表明,铁皮石斛PRC2复合体包含5个成员:E(z)同源基因DcCLF和DcSWN、Su(z)12基因DcEMF2、ESC基因DcFIE、p55基因DcMSI1,且这5个成员间的互作关系基本符合模式植物的互作模式,也存在物种特异性,表明PRC2复合体在进化中既有保守性也有特殊性。PRC2核心成员在铁皮石斛根、茎、叶、花蕾、成花中均有表达,但不同基因的表达存在组织差异性。同时,PRC2成员响应不同的环境和病害胁迫:DcCLF受低温、高温和脱水等各种环境胁迫的显著诱导;DcMSI1和DcEMF2在齐整小核菌侵染下表达明显上调,而DcSWN在灰葡萄孢霉菌侵染下受诱导程度最大。铁皮石斛PRC2复合体在生长发育和胁迫应答中发挥重要作用,可为分子辅助育种提供关键靶标基因。 相似文献
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Genes for resistance to powdery mildew in European barley cultivars registered in the Czech Republic from 2011 to 2015 下载免费PDF全文
Antonín Dreiseitl 《Plant Breeding》2017,136(3):351-356
Barley (Hordeum vulgare) is cultivated on 49.1 million hectares worldwide with 50.2% of the area located in Europe. Powdery mildew, caused by Blumeria graminis f. sp. hordei (Bgh), occurs wherever barley is grown. Cultivar resistance plays an important role in global barley production, especially in parts of Europe where high concentrations of both spring and winter types are grown. The aim of this report was to postulate specific resistance genes in barleys from nine European countries registered in the Czech Republic from 2011 to 2015. Thirty‐five spring cultivars and 27 winter barleys were tested with 56 diverse Bgh isolates. Twenty‐five known resistance genes were postulated, and unknown genes were detected in Sandra, Saturn and Zeppelin. Unidentified specific resistance genes were also present in winter hybrids Hobbit and Wootan. Spring cultivars Arthur and Francin consisted of three and two genotypes, respectively. Resistance gene mlo was present in 26 spring cultivars, and the proportion of cultivars with this gene increased from 62.9% in 2006–2010 to 75.7% in 2011–2015. The gene Mlp1 was identified for the first time in German winter cultivar Saturn. Five spring cultivars registered in Slovakia were included in the tests. All the cultivars that were tested contained one or more specific resistance genes to powdery mildew. Adaptability of the pathogen and possibilities for breeding winter barleys are discussed. 相似文献
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为研究局地尺度高寒草原土壤真菌多样性对地上植物多样性与生产力关系的调控作用,本研究以青海省湟源县局地尺度高寒草原为研究对象,对不同植物多样性梯度的地上生产力、土壤理化性状和土壤真菌多样性等指标进行调查、采样和分析。通过偏回归分析(partial least squares regression,PLSR)、方差分解分析(variance partitioning analysis,VPA)、构建结构方程模型(structural equation model,SEM)等分析手段,分析了土壤真菌多样性对地上植物多样性与生产力关系的调控作用。结果表明,高寒草原植物多样性与地上生产力存在显著线性正相关关系;土壤真菌多样性分别与植物多样性和地上生产力呈显著正相关关系;通过PLSR,VPA,SEM等方法控制土壤非生物因子效应后,真菌多样性与二者的显著正相关关系仍存在。综上所述,局地尺度下青海省湟源县高寒草原土壤真菌多样性是调控地上植物多样性与生产力正向关系的关键生物因子。 相似文献